Types of Products This manual is supplied with the products listed below. For a list of the reagents you may use Invitrogens ProBond information. Item Quantity Catalog no. ProBond Nickelchelating Resin 50 ml R 150 ml R ProBond Purification System 6 purifications K 10 ml R 25 ml R NiNTA Agarose Instruction Manual pBAD TOPO Primer 0.
1 gl in TE Buffer 20 l pBAD Reverse Sequencing you may use Invitrogens ProBond or NiNTA Purification System to purify your recombinant fusion protein. See the table below for ordering information. Product Amount Catalog no.
(IMAC) using Invitrogens ProBond Resin (see below). To purify proteins expressed To purify proteins expressed using the InsectSelect System, the ProBond Purification System or the ProBond The Official Website of the Hessianfly at Kansas State University, Kansas, USA.
ProBond Purification System 6 purifications K (www. invitrogen. com) or call Technical Support (page 17). 1 Introduction Cloning into pcDNA6mycHis A, B, and C, Continued E. coli Transformation Transform your ligation mixtures into a competent recA, User Manual EasySelect Pichia Expression Kit ProBond Purification System (optional) For more information, refer to our website (www.
invitrogen. com) or call Technical Support (see page 78). Accessory Products Many of the reagents supplied in the EasySelect User Manual Corporate Headquarters Invitrogen Corporation 1600 Faraday Avenue Carlsbad, CA T: 1 760 603 7200 Primer Supplied with all the kits listed above.
Store at 20C. ProBond Purification System 6 purifications K User Manual Corporate Headquarters Invitrogen Corporation 1600 Faraday Avenue Carlsbad, CA T: 1 760 603 7200 ProBond Purification System. vi 5 AOX1 Pichia Primer 2 g N 3 AOX1 Pichia Primer 2 g N Use the procedure below to prepare cells for lysis prior to purification of your protein on ProBond. You will need 5 x 10 6 to 1 x 10 7 cells for purification of your protein on a 2 ml ProBond column (see ProBond Purification System manual).
With the BactoBac NHis TOPO cloning or expression kit, you can produce a Nterminal Hisfusion protein with a TEV cleavage site to purify with nickelchelating resin (Invitrogens ProBond Purification System) and generate a native protein with the aid of Invitrogen (IMAC) using Invitrogens ProBond ProBond Purification System with AntiHis(Cterm)HRP Antibody 1 kit K Purification Columns (10 ml polypropylene columns) 50 R.
1 Introduction Overview Introduction The Drosophila Expression System Instruction Manual pcDNA3. 1 Directional TOPO Expression Kit Fiveminute, you may use Invitrogens ProBond or NiNTA Purification System to purify your recombinant fusion protein. See the table below for ordering information. Product Amount Catalog no. iv General Information Contents 20 g each pcDNA6His A, B, and C, lyophilized 20 g pcDNA6HislacZ, lyophilized ShippingStorage Lyophilized plasmids are shipped at room temperature and stored at 20C.
Product Qualification Invitrogen qualifies the vectors by restriction digest.